O-Propargyl-puromycin, Acetate salt
Catalogue Number: NU-931-05-JEN
| Manufacturer: | Jena Bioscience |
| Shelf Life: | 12 months |
| Physical state: | solid |
| Type: | Conjugation Reagent |
| Shipping Condition: | Blue Ice |
| Unit(s): | 0.5 mg |
Description
Description: Liu et al.[1] reported a non-radioactive alternative to analyze newly synthesized proteins in cell culture and whole organisms that is based on O-Propargyl-puromycin, an alkyne analog of puromycin. O-Propargyl-puromycin is cell-permeable and incorporates into the C-terminus of translating polypeptide chains thereby stopping translation. The resulting truncated C-terminal alkyne labeled proteins can subsequently be detected via Cu(I)-catalyzed click chemistry that offers the choice to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks or a fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging. In contrast to Azidohomoalanine (AHA) or Homopropargylgycine (HPG) based non-radioactive methionine analog-approaches, methionine free-medium is not required for O-Propargyl-purmoycin-based monitoring of nascent protein synthesis. Presolski et al.[4] and Hong et al.[5] provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.
Additional Text
Application Notes
Protein synthesis monitoring in cell culture and whole organisms[1,2]
Solubility
DMSO, PBS (up to 50 mM tested) pH adjusted to 5.0
Smile String
N(c1ncnc2c1ncn2[C@@H]1O[C@@H]([C@@H](NC(=O)[C@H](Cc2ccc(cc2)OCC#C)N)[C@H]1O)CO)(C)C
NU-931-05 MSDS
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