Biotin-14-dCTP
Catalogue Number: NU-956-BIO14-S-JEN
| Manufacturer: | Jena Bioscience |
| Shelf Life: | 12 months |
| Ph: | 7.5 +/-0 |
| Physical state: | Solution |
| Type: | Labelled Nucleotides |
| Shipping Condition: | Blue Ice |
| Unit(s): | 200 ul |
Description
Description: Biotin-14-dCTP is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dCTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal substrate properties for Nick Translation are ensured by a 14-atom linker attached to the N4 position of cytosine. For PCR incorporation experiments e.g. with Taq polymerase Biotin-11-dCTP (#NU-809-BIOX) or Biotin-16-dCTP (#NU-809-BIO16) are recommended whose Biotin moiety is attached to C5 position of cytidine via a 11-atom or 16-atom linker, respectively. Recommended Biotin-14-dCTP/dCTP ratio for Nick Translation: 50% Biotin-14-dCTP/ 50% dCTP Please note: The optimal final concentration of Biotin-14-dCTP may very depending on the application and assay conditions. For optimal product yields and high incorporation rates an individual optimization of the Biotin-14-dCTP/dCTP ratio is recommended.
Additional Text
Concentration
1.0-1.1 mM
Application Notes
Incorporation into DNA/cDNA by - Nick Translation with DNAse I/ DNA Polymerase I [1] & in-house data - Primer Extension with Klenow fragment [2]
Short Description
Bio-14-dCTP, Triethylammonium salt
Smile String
OP(=O)(O)OP(=O)(O)OP(=O)(O)OC[C@H]1O[C@@H](n2ccc(nc2=O)NCCCCCCNC(=O)CCCCCNC(=O)CCCC[C@@H]2SC[C@@H]3NC(=O)N[C@H]23)C[C@@H]1O
Molecular Weight
905.78 g/mol (free acid)
Molecular Formula
C31H54N7O16P3S (free acid)
NU-956-BIO14-S MSDS
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