C3C antibody (TRITC)

Catalogue Number: ORB20829-BOR

Manufacturer:	Biorbyt
Physical state:	Lyophilized
Type:	Polyclonal Primary Antibody - Conjugated
Shipping Condition:	Blue Ice
Unit(s):	1 ml
Host name:	Pig
Clone:
Isotype:	IgG
Immunogen:
Application:	IE, DB

Overview
Datasheets

Additional Text

Concentration

1 mg/ml

Uniprot ID

P01024

Short Description

Tetramethylrhodamine isothiocyanate-conjugated IgG fraction of polyclonal swine antiserum to C3c fragment of human complement factor C3. C3c is the major fragment resulting from C3 cleavage by C3 convertase and factor I. It is composed of an intact beta chain bound to two fragments of the alpha chain. C3c is isolated and purified from pooled normal human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Note

For research use only.

Antibody Clonality

Polyclonal

Application Notes

The antiserum does not cross-react with any other component of human plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.Conjugation Notes: A proprietary technique for the binding to TRITC is used, followed by several purification steps to remove free reactants and protein aggregates. After each step activity and specificity are tested in a variety of techniques. The conjugate is lyophilized to assure stability and long shelf life. Tetramethylrhodamine isothiocyanate isomer R. It has an orange-red fluorescence. Excitation: 554 nm, emission: 573 nm. To avoid nonspecific background staining, specially synthesized and exceptionally pure crystalline isomer R has been used instead of the usual racemic mixture. Although its fluorescence efficiency is less than of FITC, TRITC conjugates have the advantage of significantly less photo bleaching. This facilitates their use in quantitative cell- counting procedures.Experiment Notes: The IgG (7S) fraction is isolated and purified from the antiserum and contains the bulk of the defined antibody specificity. It is free of other serum proteins as tested by immunoelectrophoresis and double radial immunodiffusion.

Storage Note

The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

ORB20829 Datasheet

Price

£319.00

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