Secretory Component antibody (TRITC)

Catalogue Number: ORB21172-BOR

Manufacturer:	Biorbyt
Physical state:	Lyophilized
Type:	Monoclonal Primary Antibody - Conjugated
Shipping Condition:	Blue Ice
Unit(s):	200 ug
Host name:	Mouse
Clone:	NI 194-4 (A89-039)
Isotype:	IgG1
Immunogen:
Application:	IE, DB

Overview
Datasheets

Additional Text

Uniprot ID

P01833

Concentration

0.4 mg/ml

Short Description

Tetramethylrhodamine isothiocyanate-conjugated purified monoclonal mouse antibody to human secretory component, free and bound. To identify the presence of secretory component, free or bound, in human milk, other body fluids, cell and tissue substrates and to determine its concentration in immunofluorescence staining techniques. The optimum working dilution is an assay- related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:100. These data should be interpreted as general recommendations only.

Note

For research use only.

Antibody Clonality

Monoclonal

Application Notes

The antiserum does not react with any other component of the human milk or plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.Conjugation Notes: Conjugation is carried out using a proprietary technique for the binding of TRITC, followed by several purification steps. After each step activity and specificity are tested in a variety of techniques. No foreign protein has been added. The conjugate is lyophilized to assure stability and long shelf life. Tetramethylrhodamine isothiocyanate isomer R. It has an orange-red fluorescence. Excitation: 554 nm, emission: 573 nm. To avoid nonspecific background staining, specially synthesized and exceptionally pure crystalline isomer R has been used instead of the usual racemic mixture. Although its fluorescence efficiency is less than of FITC, TRITC conjugates have the advantage of significantly less photo bleaching.

Storage Note

The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4°C, not refrozen, and preferably used the same day.

ORB21172 Datasheet

Price

£494.00

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