Papilloma Virus, Human, Type 16 Capsid L1 (hPV) (MaxLight 650)
Catalogue Number: P3105-15A-ML650-USB
| Manufacturer: | United States Biological |
| Physical state: | Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650. |
| Type: | Monoclonal Primary Antibody - Conjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Mouse |
| Clone: | 9L396 |
| Isotype: | IgG2a, kappa |
| Immunogen: | Beta galactosidase-L1 fusion protein. |
| Application: | IP, WB, IHC |
Description
Description: MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. Specific types of HPV have been shown to be associated with human cervical cancer. One of these types, HPV16, has been shown to be present in the majority of cervical dysplasias and squamous cell carcinomas studied. Research has also shown that the expression of the HPV16-E7 gene is both sufficient and necessary for cellular transformation in vitro. The immunological detection of HPV types in human samples has been hindered by the lack of an in vitro culture system for the propagation of viruses and viral antigens. Although antibodies that were generated against the bovine papillomavirus capsid antigen (BPV-L1 ORF) are cross-reactive to HPV, these antibodies are of limited use because they do not discriminate among HPV types and have limited sensitivity as compared to standard light microscopic criteria for HPV infection. With bacterial expression systems, fusion proteins of HPV-encoded gene products can be generated and these, in turn, used to develop HPV type specific antibodies. The use of these type specific antibodies to study HPV protein expression may expand understanding of the role of HPV proteins in the pathogenesis of condylomas, dysplasias, and carcinomas of the genital tract.
Additional Text
Specificity
Recognizes HPV Type 16, L1 (major capsid protein) at ~41kD.
Purification
Protein A purified
Antibody Clonality
Monoclonal
P3105-15A-ML650 Datasheet
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