Phosphotyrosine (MaxLight 650)
Catalogue Number: P4078-01A-ML650-USB
| Manufacturer: | United States Biological |
| Physical state: | Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™650. |
| Type: | Monoclonal Primary Antibody - Conjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Mouse |
| Clone: | 5J256 |
| Isotype: | IgG2b |
| Immunogen: | Human phosphotyrosine coupled to carrier protein. |
| Application: | ICC, IP, WB, IHC, FLISA |
Description
Description: MaxLight™ 650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. Protein tyrosine phosphorylation has been the subject of intense investigation for over two decades. Tyrosine phosphorylation is catalyzed by members of the tyrosine kinase superfamily. To date, approximately 90 members of this superfamily have been identified in the human genome. Levels of phosphorylation of tyrosine residues within cellular proteins are observed to increase in response to growth factors, cytokines, insulin, extracellular matrix components and other stimuli. Tyrosine phosphorylation has several effects, including the regulation of enzyme activity, as well as the localization of enzyme activity through the generation of docking sites for proteins bearing SH2 domains.
Additional Text
Specificity
Recognizes human phosphorylated tyrosine residues. Does not crossreact with phosphorylated threonine or phosphorylated serine residues. Binding to phosphorylated tyrosine residues is inhibited by divalent cations at concentrations >1mM and salt concentrations >0.2M.
Purification
Affinity Purified
Antibody Clonality
Monoclonal
P4078-01A-ML650 Datasheet
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