PAX-8 (Paired box protein 8)
Catalogue Number: PAX008-0.5-EXA
| Manufacturer: | ExAlpha |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | Paired box protein 8 |
| Shipping Condition: | RT |
| Storage Condition: | 2-8°C |
| Unit(s): | 0.5 ml |
| Host name: | Rabbit |
| Clone: | ZR1 |
| Isotype: | IgG |
| Immunogen: | |
| Application: | IHC-P, IHC-Fr |
Description
Description: PAX-8 is expressed in the thyroid, non-ciliated mucosal cells of the fallopian tubes and simple ovarian inclusion cysts, but not normal ovarian surface epithelial cells. The antibody against PAX-8 is well suited for the detection of the protein in ovarian clear cell carcinoma. The cellular localization is nuclear. PAX-8 is expressed in the thyroid (and associated carcinomas), non-ciliated mucosal cells of the fallopian tubes and simple ovarian inclusion cysts, but not normal ovarian surface epithelial cells. PAX-8 is expressed in a high percentage of ovarian serous, endometroid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas. Studies have also found PAX-8 expression in renal tubules as well as renal carcinoma, nephroblastoma and seminoma. Over 98% of clear cell RCCs, 90% of papillary RCCs, and 95% of oncocytomas were positive for PAX-8, frequencies which are similar or better than for PAX-2. Normal lung and lung carcinomas do not express PAX-8. Similarly, the absence of expression of PAX-8 in breast and other non-GYN carcinomas other than those primary to the thyroid indicates that PAX-8 is an important new marker of ovarian cancer and a useful marker for the differential diagnoses in lung and neck tumors, or tumors at distant sites where primary lung carcinoma or thyroid carcinoma are possibilities. PAX-8, combined with organ system-specific markers such as uroplakin, mammaglobin, and TTF-1 can be a very useful panel to differentiate the primary site of invasive micro-papillary carcinomas of ovary from those of the bladder, lung, and breast. The ZR1 antibody does not react with pancreatic neuroendocrine tumors, thymic tumors, and lymphocytes.
Additional Text
Gene ID
7849
Gene Name
PAX8
Uniprot ID
Q06710
Antibody Clonality
Monoclonal
Storage Note
2-8°C
Application Notes
IHC (C,P) Staining procedures: Use formalin-fixed and paraffin-embedded sections. Retrieval conditions: Pretreatment of deparaffinized tissue with heat-induced epitope retrieval (EDTA, pH9.0) is recommended. Detection methods: Polymer anti-mouse/rabbit Ig detection system. Working dilution: 1:50-100; Positive Control: Ovarian serous carcinoma. Cellular Localization: Nuclear.
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