Pfu-X Polymerase, Proofreading DNA polymerase for highest accuracy, Pyrococcus furiosus, recombinant, E. coli
Catalogue Number: PCR-207L-JEN
| Manufacturer: | Jena Bioscience |
| Shelf Life: | 12 months |
| Physical state: | Liquid |
| Type: | DNA Polymerases |
| Shipping Condition: | Blue Ice |
| Unit(s): | 500 units |
| Application: | PCR |
Description
Description: Pfu-X Polymerase is the ideal choice for applications where the efficient amplification of DNA with highest fidelity is required. The enzyme is a genetically engineered Pfu DNA polymerase, but showing a 2-fold higher accuracy and an increased processivity, resulting in shorter elongation times.The enzyme catalyzes the polymerization of nucleotides into duplex DNA in 5' to 3' direction but does not possess a 5' to 3' exonuclease replacement activity. Its inherent 3' to 5' exonuclease proofreading activity results in a greatly increased fidelity of DNA synthesis compared to Taq polymerase. Pfu-X Polymerase-generated PCR fragments are blunt-ended. The enzyme is highly purified and free of bacterial DNA.Fidelity of the enzyme:Pfu-X Polymerase is characterized by a 50-fold higher fidelity compared to Taq polymerase and a 2-fold higher fidelity compared to standard Pfu polymerase.ERPfu-X Polymerase = 0.25 x 10-6The error rate (ER) of a PCR reaction is calculated using the equation ER = MF/(bp x d), where MF is the mutation frequency, bp is the number of base pairs of the fragment and d is the number of doublings(2d = amount of product / amount of template).
Additional Text
Concentration
2.5 unit/ul
PCR-207L MSDS
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