qPCR MultiplexMaster, Master mix for multiplex real-time PCR

Catalogue Number: PCR-321S-JEN

Manufacturer:Jena Bioscience
Shelf Life:12 months
Physical state:Liquid
Type:qPCR Mixes
Shipping Condition:Blue Ice
Unit(s): 2 x 1.25 ml
Application: PCR


Description: qPCR MultiplexMaster is designed for quantitative real-time analysis of DNA samples using Dual Labeled Fluorescent Probes, e.g. TaqMan®, Molecular Beacons or FRET probes. The master mix is specially optimized for setting-up multiplex assays with ≥4 target sequences in a single tube. The system overcomes multiplex limitations of conventional qPCR probe mixes combining an above-average robustness for a multitude of known PCR inhibitors with an excellent sensitivity for amplification of lowest template amounts. The 2x concentrated master mix contains all reagents required for qPCR (except template and primer/probe sets) including a highly processive antibody-inhibited hot-start polymerase and ultra-pure dNTPs. The mixes provide an extremely stringent automatic hot-start allowing reaction set-up and temporary storage at room temperature prior to PCR. The reaction chemistry of the mix is optimized for block-based PCR instruments. The mix can also be used with ROX reference dye (#PCR-351) in PCR instruments that are compatible with the evaluation of the ROX signal

Additional Text


2x conc.

Additional Information

Dual-labeled DNA probes: Real-time PCR technology based on dual-labeled DNA probes provides a high sensitive and high specific PCR system with multiplexing capability. For amplification of each target sequence a set of two PCR primers and one fluorescent DNA probe that hybridizes to an internal part of the amplicon are required. The sequence of the dual-labeled DNA probe should avoid secondary structure and primer-dimer formation. Preparation of the qPCR master mix: The preparation of a master mix is crucial in quantitative PCR reactions to reduce pipetting errors. Prepare a master mix of all components except template as specified. A reaction volume of 20-50 µl is recommended for most real-time instruments. Prepare 13 volumes of master mix for 12 samples or a triple-set of 4 samples. Pipet with sterile filter tips and minimize the exposure of the labeled DNA probe to light. Perform the setup in an area separate from DNA preparation or analysis. No-template controls should be included in all amplifications. component||20 µlassay||50 µlassay||final conc. qPCR MultiplexMaster||10 µl||25 µl||1x each primerforward(10 µM)1)||0.6 µl||1.5 µl||300 nM each primerreverse(10 µM)1)||0.6 µl||1.5 µl||300 nM each dual-labeled probe(10 µM)2)||0.4 µl||1 µl||200 nM template DNA||x µl||x µl||


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