qPCR MultiplexMaster, Master mix for multiplex real-time PCR

Catalogue Number: PCR-321S-JEN

Manufacturer:Jena Bioscience
Shelf Life:12 months
Physical state:Liquid
Type:qPCR Mixes
Shipping Condition:Blue Ice
Unit(s): 2 x 1.25 ml
Application: PCR


Description: qPCR MultiplexMaster is designed for quantitative real-time analysis of DNA samples using Dual Labeled Fluorescent Probes, e.g. TaqMan®, Molecular Beacons or FRET probes. The master mix is specially optimized for setting-up multiplex assays with ≥4 target sequences in a single tube. The system overcomes multiplex limitations of conventional qPCR probe mixes combining an above-average robustness for a multitude of known PCR inhibitors with an excellent sensitivity for amplification of lowest template amounts. The 2x concentrated master mix contains all reagents required for qPCR (except template and primer/probe sets) including a highly processive antibody-inhibited hot-start polymerase and ultra-pure dNTPs. The mixes provide an extremely stringent automatic hot-start allowing reaction set-up and temporary storage at room temperature prior to PCR. The reaction chemistry of the mix is optimized for block-based PCR instruments. The mix can also be used with ROX reference dye (#PCR-351) in PCR instruments that are compatible with the evaluation of the ROX signal