Catalogue Number: PCR-351-JEN
|Shelf Life:||12 months|
|Shipping Condition:||Blue Ice|
Description: ROX reference dye is optimized for block-based real-time PCR instruments that are compatible with the evaluation of the ROX reference signal. The dye does not take part in the PCR reaction but allows to normalize for non-PCR related signal variation and provides a baseline in multiplex reactions. The dye is supplied as 50x concentration.
Preparation of the qPCR master mix: Preparation of a master mix is crucial in quantitative PCR reactions to reduce pipetting errors. Vortex the ROX reference dye thoroughly before use. Add ROX reference dye to your PCR assay as indicated below. final ROXconcentration||20 µl PCR assay||50 µl PCR assay low ROX assay50 nM||0.04 µl||0.1 µl high ROX assay500 nM||0.4 µl||1.0 µl