Catalogue Number: PCR-353-JEN
|Shelf Life:||12 months|
|Shipping Condition:||Blue Ice|
Description: Thermolabile UNG is used in real-time PCR to prevent carry-over contamination of dU-containing DNA from previous reactions. Uracyl N-Glycosylase (UNG, UDG) catalyses the release of uracil from single and double stranded uracyl-containing DNA. The resulting abasic sites are susceptible to hydrolytic cleavage at elevated temperatures. An amount of 1 unit UNG completely digests 104 to 106 copies of U-containing DNA fragments in 2 min at 50°C. Recommended assay: Add 0.2 µl (0.2 units) UNG for each 50 µl of master mix and vortex thoroughly. The preparation of a master mix is crucial in quantitative PCR reactions to reduce pipetting errors. An UNG treatment of 2 min at 50°C at the onset of thermal cycling removes uracil residues from dU-containing DNA and prevents it from serving as template. UNG is complitely heat-inactivated at temperatures above 65°C in the following initial denataration step of the PCR.