Thermolabile UNG (Uracil N-Glycosylase), Prevention of carry-over contaminations
Catalogue Number: PCR-353-JEN
| Manufacturer: | Jena Bioscience |
| Shelf Life: | 12 months |
| Physical state: | Liquid |
| Type: | qPCR Kits |
| Alias: | UNG, UDG |
| Shipping Condition: | Blue Ice |
| Unit(s): | 200 units |
| Application: | PCR |
Description
Description: Thermolabile UNG is used in real-time PCR to prevent carry-over contamination of dU-containing DNA from previous reactions. Uracyl N-Glycosylase (UNG, UDG) catalyses the release of uracil from single and double stranded uracyl-containing DNA. The resulting abasic sites are susceptible to hydrolytic cleavage at elevated temperatures. An amount of 1 unit UNG completely digests 104 to 106 copies of U-containing DNA fragments in 2 min at 50°C. Recommended assay: Add 0.2 µl (0.2 units) UNG for each 50 µl of master mix and vortex thoroughly. The preparation of a master mix is crucial in quantitative PCR reactions to reduce pipetting errors. An UNG treatment of 2 min at 50°C at the onset of thermal cycling removes uracil residues from dU-containing DNA and prevents it from serving as template. UNG is complitely heat-inactivated at temperatures above 65°C in the following initial denataration step of the PCR.
Additional Text
Concentration
1 unit/ul
PCR-353 MSDS
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations