Thermolabile UNG (Uracil N-Glycosylase)

Catalogue Number: PCR-353-JEN

Manufacturer:Jena Bioscience
Shelf Life:12 months
Physical state:Liquid
Type:qPCR Kits
Alias:UNG, UDG
Shipping Condition:Blue Ice
Unit(s): 200 units

Description

Description: Thermolabile UNG is used in real-time PCR to prevent carry-over contamination of dU-containing DNA from previous reactions. Uracyl N-Glycosylase (UNG, UDG) catalyses the release of uracil from single and double stranded uracyl-containing DNA. The resulting abasic sites are susceptible to hydrolytic cleavage at elevated temperatures. An amount of 0.1 units UNG can completely destroy up to 200 ng dU-containing DNA in 2 min at 50°C. Recommended assay: Add 0.2 ul (0.2 units) UNG for each 50 ul of master mix and vortex thoroughly. The preparation of a master mix is crucial in quantitative PCR reactions to reduce pipetting errors. An UNG treatment of 2 min at 50°C at the onset of thermal cycling removes uracil residues from dU-containing DNA and prevents it from serving as template. UNG is easily heat-inactivated at temperatures above 65°C in the following initial denataration step of the PCR.

Additional Text

Concentration

1 unit/ul

Price
108.00


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