SYBR® Green Fluorescent DNA Stain, DNA intercalation dye for real-time PCR analysis

Catalogue Number: PCR-378-JEN

Manufacturer:Jena Bioscience
Shelf Life:12 months
Physical state:Liquid
Type:Nucleic Acid Stain
Shipping Condition:Blue Ice
Unit(s): 500 ul
Application: PCR

Description

Description: SYBR® Green Fluorescent DNA Stain is a superior DNA intercalator dye specially developed for DNA analysis applications including real-time PCR (qPCR). Upon binding to DNA, the non-fluorescent dye becomes highly fluorescent while showing no detectable inhibition to the PCR process. The dye is extremely stable both thermally and hydrolytically, providing convenience during routine handling.

Additional Text

Concentration

100 uM

Additional Information

SYBR® Green Fluorescent DNA Stain is supplied as 100 µM concentration. Vortex SYBR® Green Fluorescent DNA Stain thoroughly prior to its use. An SYBR® Green concentration of 0.5-1.0 µM in the final assay is recommended. Add SYBR® Green Fluorescent DNA Stain as indicated in the table below per assay. Please note that the preparation of a master mix may be crucial in quantitative PCR reactions to reduce pipetting errors. Select the optical setting for SYBR® Green or FAM on the detection instrument. final SYBR® Green concentration||20 µl PCR assay||50 µl PCR assay 0.5 µM||0.1 µl||0.25 µl 1.0 µM||0.2 µl||0.50 µl SYBR® is a registered trademark of Invitrogen Corporation, Carlsbad, California, USA.