Catalogue Number: PCR-378-JEN
|Shelf Life:||12 months|
|Type:||Nucleic Acid Stain|
|Shipping Condition:||Blue Ice|
Description: SYBR® Green Fluorescent DNA Stain is a superior DNA intercalator dye specially developed for DNA analysis applications including real-time PCR (qPCR). Upon binding to DNA, the non-fluorescent dye becomes highly fluorescent while showing no detectable inhibition to the PCR process. The dye is extremely stable both thermally and hydrolytically, providing convenience during routine handling.
SYBR® Green Fluorescent DNA Stain is supplied as 100 µM concentration. Vortex SYBR® Green Fluorescent DNA Stain thoroughly prior to its use. An SYBR® Green concentration of 0.5-1.0 µM in the final assay is recommended. Add SYBR® Green Fluorescent DNA Stain as indicated in the table below per assay. Please note that the preparation of a master mix may be crucial in quantitative PCR reactions to reduce pipetting errors. Select the optical setting for SYBR® Green or FAM on the detection instrument. final SYBR® Green concentration||20 µl PCR assay||50 µl PCR assay 0.5 µM||0.1 µl||0.25 µl 1.0 µM||0.2 µl||0.50 µl SYBR® is a registered trademark of Invitrogen Corporation, Carlsbad, California, USA.