Direct WGA Kit

Catalogue Number: PCR-382S-JEN

Manufacturer:Jena Bioscience
Shelf Life:12 months
Type:DNA Amplification Kits
Shipping Condition:Blue Ice
Unit(s): 20 reactions


Description: Direct WGA Kit is a complete system for whole genome amplification from various tissues or samples directly without DNA purification processes. Very little amount of samples, several miligram or microliter volume, are required for the direct WGA. About 10 μg DNA products could be obtained in a standard reaction. The enzyme mix and buffer system are designed to tolerate against most amplification inhibitors found in crude samples. Phi29 DNA polymerase, the major polymerization enzyme of this kit, isothermally amplifies the genomic DNAs included in the samples with multiple displacement mechanism. Phi29 DNA polymerase could produce DNA strand up to 70 kb long with high fidelity. All required components including enzymes, buffers, dNTPs, random primers, and sample pretreatment reagents are supplied in this kit. The amplified DNA products could be applied for successive PCR, genotyping, and library construction. Fast and uniform amplification across entire genomeMultiple Displacement Amplification by Phi29 DNA polymeraseDirect WGA from Whole blood, animal tissues, plant leaves and seeds, clinical & forensic sample [Saliva, Buccal swab, Hair root, Blood stain (toilet paper or paper)]

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Application Notes

Genotype analysisPCR and real-time PCRConstruction of genomic library