RNase Inhibitor - glycerol-free, rat lung, recombinant, E. coli
Catalogue Number: PCR-399-10KU-JEN
| Manufacturer: | Jena Bioscience |
| Physical state: | Liquid |
| Type: | Ribonuclease Inhibitors |
| Shipping Condition: | Blue Ice |
| Unit(s): | 10000 units |
| Application: | AP, RT-PCR, PCR, cDNASyn |
Description
Description: RNase Inhibitor - glycerol-free is recommended for use in freeze drying applications where glycerol must be avoided. RNase Inhibitor, a recombinant protein of rat lung RNase Inhibitor expressed in E. coli , effectively inhibits a broad spectrum of eukaryotic RNases, including RNase A, B and T2. It inhibits RNases by binding noncovalently in a 1:1 ratio with high affinity (4 x 10-14M). In addition, RNase Inhibitor shows no inhibition of DNA polymerase or reverse transcriptase activity and is used in cDNA synthesis and in one-step RT-PCR reactions for protection against RNase contamination.
Additional Text
Concentration
40 unit/ul
Application Notes
RNase Inhibitor should be applied in a working concentration of 10-20 units per 20 µl reaction volume. The application of RNase Inhibitor is recommended for:cDNA synthesis / RT-PCRIn vitro transcription/translationRNA purificationRNA protection assaysseparation and identification of specific ribonuclease activities applications where the integrity of RNA is essential
Storage Note
store at -80 °C storage at -20 °C for 6 months possible|see Certificate of Analysis, stored at -80 °C 6 months from delivery date if stored at -20 °C|avoid freeze/thaw cycles
PCR-399-10KU Datasheet
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