SCRIPT Reverse Transcriptase, Reverse Transcriptase with increased thermal stability

Catalogue Number: PCR-505S-JEN

Manufacturer:Jena Bioscience
Shelf Life:12 months
Physical state:Liquid
Type:Reverse Transcriptases
Shipping Condition:Blue Ice
Unit(s): 20000 units
Application: PCR

Description

Description: SCRIPT Reverse Transcriptase is a genetically engineered version of M-MLV Reverse Transcriptase (M-MLV RT) with eliminated RNase H activity and increased thermal stability. The enzyme is a RNA-directed DNA polymerase that synthesizes a complementary DNA strand initiating from a primer using single-stranded RNA or DNA as template. Its enhanced thermal stability in combination with the deactivated RNase H activity results in an increased specificity, higher cDNA yield and an improved efficiency for full length cDNA synthesis compared with standard M-MLV RT. The enzyme is recommended for synthesis of cDNA from 100 bp up to 10 kb length.

Additional Text

Application Notes

Extremely sensitive and highly specific RT-PCR, synthesis of highly structured and long cDNA fragments, DNA labelling.

Concentration

200 unit/ul