Alpaca-pHast (VHH) [PH-05] (Far Red)

Catalogue Number: PH-05F-ATS

Manufacturer:	Advanced Targeting Systems
Type:	Polyclonal Secondary Antibody - Conjugated
Shipping Condition:	Blue Ice
Storage Condition:	2-8°C
Unit(s):	1 mg, 250 ug, 100 ug
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Overview
Datasheets

Description

Description: pHast Conjugates are one of our pHastest tools for quantitative testing of your primary antibody’s specificity, binding, and internalization, providing results in 1 day.

Fluorescence intensity increases over time, indicating internalization of the pHast conjugate. Data generated using an IncuCyte SX5.

How It Works

The pHast conjugate binds to your primary antibody via a secondary antibody cross-linked to a pH-dependent fluorescent reporter. This fluorescent reporter will increase intensity as the pH of its surroundings becomes more acidic, as evident when exposed to the environment inside a cell. pHast conjugates are fluorescent plate reader compatible and can be used to illuminate your lead antibody candidates in a 96-well plate format.

All of our pHast Conjugates are available with these fluorescent dyes.

The pHast RED has an excitation wavelength of 532 nm with an emission maxima at 560 nm.

The pHast FAR-RED has an excitation wavelength of 643 nm with an emission maxima at 660 nm.

The pHast GREEN version has an excitation wavelength of 453 nm with an emission maxima at 522 nm.

The pHast BLUE has an excitation wavelength of 362 nm with an emission maxima at 452 nm.

PH-05F Datasheet

Price

£4,718.00

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