Human PLAU/uPA Protein (active form) (PLA-HM101)
Catalogue Number: PLA-HM101-KAT
PLA-HM101 Image
PLA-HM101 Image
PLA-HM101 Image
Human PLAU (activated by trypsin) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
PLA-HM101 Image
Measured by its ability to cleave a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC). The specific activity is >2000 pmol/min/ug (QC Test).
| Manufacturer: | Kactus |
| Shelf Life: | 12 months |
| Molecular Weight: | The protein has a predicted MW of 17.9 kDa (long chain A), 29.2 kDa (chain B) and 15.3 kDa (N-terminal fragment). The protein migrates to 30-38 kDa and 17 kDa based on Bis-Tris PAGE result. |
| Physical state: | Lyophilized |
| Type: | Proteases |
| Alias: | PLAU; Urokinase; ATF; UPA; URK; u-PA; BDPLT5; QPD |
| Host Cell: | HEK293 cells |
| Shipping Condition: | RT |
| Unit(s): | 100 ug, 500 ug |
| Application: | WB |
Description
Description: Plasminogen activator, urokinase (uPA) is a secreted serine protease, cleaving the sequence Cys-Pro-Gly-Arg560-Val561-Val-Gly-Gly-Cys in plasminogen to form plasmin. Dysregulation of PLAU is often accompanied by various cancers and PLAU inhibition could suppress tumor growth. Collectively, PLAU is necessary for tumor progression and can be a diagnostic and prognostic biomarker in HNSCC.
Additional Text
Protein Tag
HIS Tag
UniProt ID
P00749-1
Storage Note
-20 to -80°C for 12 months as supplied from date of receipt.; -80°C for 3 months after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Short Description
Recombinant Human PLAU/uPA Protein (active form) is expressed from HEK293 with His tag at the C-Terminus.It contains Ser21-Leu431, which consists of two chains: Long chain A (Ser21-Phe177) and chain B (Ile179-Leu431). The long chain A is further cleaved to yield a short chain A (Lys156-Phe177) and N-Terminus fragment (Ser21-Lys155).
Biological Activity
Immobilized Human PLAU, His Tag at 0.5Oeg/ml (100Oel/Well) on the plate. Dose response curve for Human uPAR, hFc Tag with the EC50 of 10.3ng/ml determined by ELISA. The affinity constant of 5.87 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Endotoxin
Less than 1 EU per µg by the LAL method.
PLA-HM101 Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations