C-HCV, Combined Hepatitis C Virus, NS3/NS4/NS5, recombinant, E. coli
Catalogue Number: PR-1143-JEN
| Manufacturer: | Jena Bioscience |
| Shelf Life: | 12 months |
| Molecular Weight: | 70 kda |
| Physical state: | Liquid |
| Type: | Virus Proteins |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Application: | ELISA, WB |
Description
Description: The protein contains the HCV nucleocapsid, NS3, NS4, and NS5 immunodominant regions. Combined Hepatitis C Virus protein is purified by proprietary chromatographic techniques. Background: Sequences from 4 gene products (proteins) of the hepatitis C virus (HCV) were scanned by using 3 different PCR-based techniques in search of the most immunoreactive regions suitable for the development of a diagnostic test for the detection of anti-HCV in human sera. All PCR fragments were cloned with pGEX4-2T expression vector and expressed in E. coli as chimeric proteins with glutathione S-transferase. The most diagnostically relevant proteins identified in this study were then constructed into one recombinant antigen.
Additional Text
Additional Information
Specificity: Immunoreactive with sera of HCV-infected individuals.
Application Notes
Antigen in ELISA and Western blots, excellent antigen for detection of HCV with minimal specificity problems.
Storage Note
store at -20 °C|12 months|avoid freeze/thaw cycles
PR-1143 MSDS
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