Catalogue Number: PTPD527VA-1-SBI
Manufacturer: | System Biosciences |
Shelf Life: | Properly stored components are stable for 12 months from the date received |
Type: | Virus |
Shipping Condition: | Dry Ice |
Unit(s): | >1x10^6 IFUs |
Description:
Overview
Faster insights into protein function in vivo
Quickly and directly study the role a protein plays in vivo with SBI’s Piranha Targeted Protein Degradation System, Electroporation-ready mRNA. After electroporating both the Piranha mRNA and an antibody specific for your protein-of-interest into target cells, you can start seeing degradation of the protein-of-interest in as little as 1 hour*. Piranha Electroporation-ready mRNA is available in untagged, RFP-tagged, or GFP-tagged versions (also available as Pre-packaged Lentivirus or HEK293T Stable Cell Line).
While studying protein function in vivo often involves knocking down expression using siRNA or knocking out the gene via CRISPR/Cas9, these approaches are indirect and often imperfect. siRNA can reduce or eliminate protein expression, but if the protein turn-over rate is slow then it can take days to see a reduction in function. Simply knocking out the gene coding for the protein-of-interest is not always possible, limits the type of studies you can do, and is incompatible with essential genes. To give researchers another faster option, SBI developed the Piranha Targeted Protein Degradation System.
*Based on published data by Clift et al. A Method for the Acute and Rapid Degradation of Endogenous Proteins. Cell. 2017 Dec 14;171(7):1692-1706.e18. doi: 10.1016/j.cell.2017.10.033. Degradation rate of target protein and resulting phenotypic effects will depend on the specific protein being targeted and influenced by protein degradation pathway and any compensatory mechanisms. An initial time course experiment to find optimal conditions to assay for protein knockdown and phenotypes is highly recommended.
How It Works
Targeted Protein Degradation with Piranha
SBI’s Piranha Targeted Protein Degradation System is based on the TRIM21 pathway found in most mammalian cells1. Like TRIM21, the Piranha protein binds to conserved regions of an antibody, targeting the antibody—and the bound antigen—for degradation by the proteasome.
To use Piranha Electroporation-ready mRNA for targeted protein degradation, simply electroporate the Piranha mRNA and a validated antibody specific for the protein-of-interest into target cells, wait an appropriate amount of time, and then observe the phenotypic outcome. Because the rate of protein degradation is based upon many factors, we recommend performing initial experiments to empirically determine the degradation rate of your target protein and the optimal time for phenotypic observation.