Description: The Vazyme HiScript II One Step qRT-PCR Probe Kit is specially designed for qPCRs that directly use RNA as templates. The reverse transcription and PCR can be finished in one tube, significantly reducing pipetting procedures and the risk of contamination. The HiScript II Reverse Transcriptase and hot-start Champagne Taq DNA Polymerase contained this kit enables high-sensitive total RNA detection (as little as 1 pg).
The HiScript II One Step qRT-PCR Probe Kit is a master mix system. The 2× One Step Q Probe Mix contains an optimized buffer and dNTP/dUTP mix and is suitable for high-sensitive detection systems based on fluorescence labelled probes (i.e. TaqMan®).
• Detectable for templates with single-digit copies or 0.1 pg total RNA
• Support multi-probe detection
HiScript® II One Step qRT-PCR Probe Kit (Q222) has a wider range of linearity and higher sensitivity
A. Taking 1 ng-0.1 pg total RNA from Hela cells as template, amplify RFLPO gene. The linearity range reaches to 5 orders of magnitude. Sensitivity reaches to 0.1 pg.
B. Taking 107-10 copies of reverse-transcribed RNAs as template, amplify HCV gene. The linearity range reaches to 7 orders of magnitude. Sensitivity reaches to 10 copies.
C. Taking allantoic fluid virus RNA with the dilution of 10-1-10-7 as template, amplify special gene of Newcastle disease virus. Dilution of 10-7 can be detected stably.