Rabbit anti Mouse albumin, conjugated with Horseradish peroxidase
Catalogue Number: RAM/ALB/PO-NMB
| Manufacturer: | Nordic MuBio |
| Type: | Polyclonal Primary Antibody - Conjugated |
| Shipping Condition: | RT |
| Unit(s): | 1 ml |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | |
| Immunogen: | |
| Application: | ELISA, ICC, DB, IB |
Description
Description: Tested in immunoelectrophoresis and double radial immunodiffusion against pooled normal mouse serum and purified mouse albumin. One characteristic precipitin line is obtained against pooled normal mouse serum using different antigen/antibody concentration ratio's. Precipitin lines against normal mouse serum and purified mouse albumin give a reaction of full identity. In enzyme-immunocytochemical and immunohistochemical staining for the detection of albumin of appropriately treated cell and tissue substrates at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA) to identify and measure albumin in mouse serum or other body fluid. In electron microscopy, since the complex between the conjugated antibody and the antigen also has electron-dense properties. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Additional Text
Antibody Clonality
Polyclonal
Storage Note
The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
Application Notes
ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.
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