Rabbit anti Mouse milk specific proteins

Catalogue Number: RAM/MSP-NMB

Manufacturer:	Nordic MuBio
Type:	Polyclonal Primary Antibody - Unconjugated
Shipping Condition:	RT
Unit(s):	1 ml
Host name:	Rabbit
Clone:
Isotype:
Immunogen:
Application:	IE, IHC-P, IHC-Fr

Overview

Description

Description: In immunoelectrophoresis against mouse milk precipitation of not less than 7 different proteins may be obtained. However the number of visible precipitin lines varies among individual specimens of mouse milk. The total concentration of protein, their proportion of the different protein components change markedly during the course of lactation. There are also strain differences in the protein composition, but differences among individual animals tend to be greater. A protein detected in a mouse secretion by this antiserum is of secretory origin. However an analogous or homologous protein may be present also n other kind of secretions (e.g. saliva). Not all precipitable proteins have been identified. Known proteins include free secretory component and lactoferrin. In immunoelectrophoresis to identify the milk protein pattern, to compare individual samples and to make comparisons with serum protein patterns or those of other secretions; to identify individual components, their possible origin and significant changes in concentration. To test the purity of an isolated milk protein.

Additional Text

Antibody Clonality

Polyclonal

Storage Note

The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.

Application Notes

Precipitation assays. In immunoelectrophoresis use 2 µl milk or equivalent against 120 µl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 µl antiserum in 3 mm diameter center well and 2 µl milk samples (neat and serially diluted in 2 mm diameter peripheral wells. Different bleedings of the immunized animals are pooled to obtain a broad spectrum balanced against the varying concentrations of the individual protein components in individual milk samples. Immunohistochemical application: For immunostaining, paraffin-embedded sections are dehydrated and antigenic epitopes are exposed by treatment with 10mM citrate buffer for 5min at 121°C using an autoclave (see reference 1).

Price

£236.00

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