Poly(A) Tailing Enzyme Testkit, in vitro Polyadenylation of (m)RNA with E. coli and Yeast Poly(A) Polymerase
Catalogue Number: RNT-004-JEN
| Manufacturer: | Jena Bioscience |
| Shelf Life: | 12 months |
| Type: | Nucleic Acid Labeling Kits |
| Shipping Condition: | Blue Ice |
| Unit(s): | 75 reactions |
Description
Description: The Poly(A) Tailing Enzyme Testkit is designed to add a poly(A) tail to the 3' end of (m)RNA transcripts generated by in vitro transcription with HighYield T7 ARCA (m)RNA Synthesis Kits (#RNT-102, #RNT-103). The crude in vitro transcription reaction mix (approx. 10-30 µg RNA) can directly be used as template. Polyadenylated (m)RNA exhibit increased stability and thus enhanced translation efficiency in transfection, microinjection or in vitro translation experiments in eukaryotic cells. The reaction is catalyzed by Poly(A) Polymerases that transfer AMP to 3'-hydroxyl ends of (m)RNA molecules. It is template-independent, requires ATP as substrate and Mg2+ or Mn2+as cofactor. The average length of attached poly(A) tails not only depends on RNA sequence, incubation time and enzyme concentration but also on the type of Poly(A) Polymerase. The Poly(A) Tailing Enzyme Testkit allows a direct comparison of E. coli and Yeast Poly(A) Polymerase to find the most suitable enzyme for a specific application. The kit contains sufficient reagents for 25 reactions, 100 µl each with 20 units E. coli Poly(A) polymerase/reaction50 reactions, 100 µl each with 600 units Yeast Poly(A) polymerase/reaction
RNT-004 MSDS
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