T7 P&L RNA Polymerase HC, Highly concentrated and modified T7 RNA Polymerase for set-up of high yield in vitro transcription reactions
Catalogue Number: RNT-018-JEN
| Manufacturer: | Jena Bioscience |
| Shelf Life: | 12 months |
| Physical state: | Liquid |
| Type: | RNA polymerases |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 ul |
Description
Description: Bacteriophage T7 RNA Polymerase is a DNA-dependent RNA polymerase (99 kDa) that catalyzes in vitro RNA synthesis from a DNA sequence containing a T7 phage promoter. The highly concentrated version and modified T7 RNA Polymerase HC is ideally suited for set-up and optimization of high yield in vitro transcription reactions. Proline 266 has been replaced by leucine (P266L) which has been associated with decreased abortive transcription[1].increased 5' homogeneity of transcripts synthesized from A-initiating phi2.5 promoter[2]increased 5' incorporation efficiency of GTP analogs[3] The provided T7 IVT Set-up Buffer, MgCl2 and DTT solutions enable testing of different reaction conditions such as nucleotide/ MgCl2 concentrations. Recommendation for assay set-up with minimal optimization: HighYield T7 P&L RNA Synthesis Kit (#RNT-201). Activity: 1 µl T7 P&L RNA Polymerase HC generates >/= 30 µg RNA in 10 min at 37°. This is comparable to 1000 U of NEB's T7 RNA Polymerase (High Concentration), #M0460T (activity assay conditions: 1x T7 IVT Set-up Buffer supplemented with 10 mM DTT, 12 mM MgCl2, 3 mM NTPs, 1 µl T7 RNA Polymerase HC and 1 µg DNA template (1.4 kpb RNA transcript) in 20 µl. RNA yield was determined with a fluorescence microplate assay.)
Additional Text
Concentration
2-2.2 ug/ul
RNT-018 MSDS
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