T7 3M RNA Polymerase HC, Highly concentrated triple mutant T7 RNA Polymerase for set-up of high yield in vitro transcription reactions
Catalogue Number: RNT-019-JEN
| Manufacturer: | Jena Bioscience |
| Shelf Life: | 12 months |
| Physical state: | Liquid |
| Type: | RNA polymerases |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 ul |
Description
Description: Bacteriophage T7 RNA Polymerase is a DNA-dependent RNA polymerase (99 kDa) that catalyzes in vitro RNA synthesis from a DNA sequence containing a T7 phage promoter. The highly concentrated triple mutant (3M) is ideally suited for the incorporation of 2'-fluoro modified NTPs. The provided T7 IVT Set-up Buffer, MgCl2 and DTT solutions enable testing of different reaction conditions such as nucleotide/ MgCl2 concentrations. Activity: 1 µl T7 3M RNA Polymerase HC generates >/= 20 µg RNA in 10 min at 37°. Activity assay conditions: 1x T7 IVT Set-up Buffer supplemented with 10 mM DTT, 12 mM MgCl2, 3 mM NTPs, 1 µl T7 3M RNA Polymerase HC and 1 µg DNA template (1.4 kpb RNA transcript) in 20 µl. RNA yield was determined with a fluorescence microplate assay.
Additional Text
Concentration
2-2.2 ug/ul
RNT-019 MSDS
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations