HighYield T7 AF555 RNA Labeling Kit, Preparation of randomly AF555-modified RNA probes by in vitro transcription with UTP-PEG5-AF555

Catalogue Number: RNT-101-AF555-JEN

Manufacturer:	Jena Bioscience
Shelf Life:	12 months
Type:	Nucleic Acid Labeling Kits
Shipping Condition:	Blue Ice
Unit(s):	20 reactions

Overview
Datasheets

Description

Description: HighYield T7 AF555 RNA Labeling Kit is designed to produce randomly AF555-modified RNA probes via in vitro transcription. Such probes are ideally suited for in situ hybridization and Northern Blot experiments. AF55 (a structural analog of Alexa Fluor® 555) is a hydrophilic dye with increased photostability compared to Cy3. UTP-PEG5-AF555 is efficiently incorporated into RNA as substitute for its natural counterpart UTP using an optimized reaction buffer and T7 RNA Labeling Polymerase Mix. 35 % UTP-PEG5-AF555 substitution typically results in an optimal balance between reaction and labeling efficiency. Individual optimization of UTP-PEG5-AF555/UTP ratio however, can easily be achieved with the single nucleotide format. The resulting AF555-modified RNA probe can subsequently be detected by fluorescence spectroscopy. The kit contains sufficient reagents for 20 labeling reactions of 20 µl each (35 % UTP-PEG5-AF555 substitution, 2.5 mM ATP, GTP, CTP, 0.2 mM UTP, 0.1 mM UTP-PEG5-AF555). A 20 µl reaction yields about 5-7 µg RNA after 30 min incubation (1 µg T7 control template, 1.4 kb RNA transcript). Yields may however vary depending on the template (promotor design, sequence length, secondary structure formation).

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Smile String

P(=O)(OP(=O)(O)O)(O)OP(=O)(O)OC[C@H]1O[C@H]([C@@H]([C@@H]1O)O)n1c(=O)[nH]c(=O)c(c1)/C=C/CNC(=O)CCOCCOCCOCCOCCOCCNC(=O)CCCCC[N+]1=C(/C=C/C=C/2C(c3c(N2CCCS(=O)(=O)O)ccc(c3)S(=O)(=O)O)(C)C)C(c2c1ccc(c2)S(=O)(=O)[O-])(C)CCCCS(=O)(=O)O

RNT-101-AF555 MSDS

RNT-101-AF555 Datasheet

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£396.00

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