HighYield T73M Aptamer Synthesis Kit (2'F-dCTP), Synthesis of 2'-Fluoro-modified RNA
Catalogue Number: RNT-302-JEN
| Manufacturer: | Jena Bioscience |
| Shelf Life: | 12 months |
| Type: | Nucleic Acid Labeling Kits |
| Shipping Condition: | Blue Ice |
| Unit(s): | 30 reactions |
Description
Description: HighYield T73M Aptamer Synthesis Kit (2'F-dCTP) is designed to produce large amounts of 2'-Fluoro-modified RNA via in vitro transcription with T73M RNA polymerase. 2'-fluoro modified RNA exhibits an increased RNA stability and is ideally suited for aptamer preparation. A modified T7 RNA polymerase (T73M RNA polymerase) ensures the efficient incorporation of 2'-fluoro modified dCTP instead of is natural counterpart CTP. The kit contains sufficient reagents for 30 reactions a 20 µl (7.5 mM ATP, 7.5 mM GTP, 7.5 mM 2'-Fluoro-dCTP, 7.5 mM UTP). An individual optimization of 2'-Fluoro-dCTP concentration can easily be achieved with the single nucleotide format. A 20 µl reaction yields about 20 µg RNA after 30 min incubation (1 µg T7 control template, 1.4 kb RNA transcript). Yields may however vary depending on the template (promotor design, sequence length, secondary structure formation).
RNT-302 MSDS
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