Human R spondin 1/RSPO1 Protein (RS1-HM101)
Catalogue Number: RS1-HM101-KAT
RS1-HM101 Image
RS1-HM101 Image
RS1-HM101 Image
RS1-HM101 Image
RS1-HM101 Image
RS1-HM101 Image
Human R spondin 1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
RS1-HM101 Image
Immobilized Human R spondin 1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-R spondin 1 Antibody, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA (QC Test).
RS1-HM101 Image
Immobilized Human R spondin 1, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human RNF34, hFc Tag with the EC50 of 0.4ug/ml determined by ELISA.
RS1-HM101 Image
Immobilized Human LRP-6, mFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human R spondin 1, His Tag with the EC50 of 4.4ug/ml determined by ELISA.
RS1-HM101 Image
Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50-for this effect is 1.0 - 10.0 ng/mL in the presence of 5 ng/mL recombinant Human Wnt Surrogate-Fc Fusion (WNT-HM23A) (QC Test).
| Manufacturer: | Kactus |
| Shelf Life: | 12 months |
| Molecular Weight: | The protein has a predicted MW of 27.3 kDa. Due to glycosylation, the protein migrates to 38-42 kDa based on Bis-Tris PAGE result. |
| Physical state: | Lyophilized |
| Type: | Other Recombinant Protein |
| Alias: | R-spondin-1; RSPO1; CRISTIN3; FLJ40906; RP11-566C13.1; RSPO; R-Spondin1 |
| Host Cell: | CHO Cells |
| Shipping Condition: | RT |
| Unit(s): | 100 ug, 500 ug |
| Application: | ELISA, WB |
Description
Description: The R-spondins are members of a superfamily of thrombospondin type 1 repeat (TSR-1)-containing proteins. The prototype member (discovered in 1971) was isolated from platelets that had been stimulated with thrombin, and was therefore designated 'thrombin-sensitive protein.
Additional Text
Gene ID
284654
Uniprot ID
Q2MKA7
Gene Name
RSPO1
Protein Tag
HIS Tag
Short Description
Recombinant Human R spondin 1/RSPO1 Protein is expressed from CHO with His tag at the C-Terminus.It contains Arg31-Ala263.
Storage Note
-20 to -80°C for 12 months as supplied from date of receipt.; -80°C for 3 months after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Biological Activity
Immobilized Human R spondin 1, His Tag at 0.5Oeg/ml (100Oel/well) on the plate. Dose response curve for Anti-R spondin 1 Antibody, hFc Tag with the EC50 of 7.1ng/ml determined by ELISA. See testing image for detail.
Endotoxin
Less than 0.05 EU per µg by the LAL method.
RS1-HM101 Datasheet
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