Sheep anti Human Vav-2 DPH
Catalogue Number: S120P-EXA
| Manufacturer: | ExAlpha |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | VAV-2; Guanine nucleotide exchange factor VAV-2, Dbl homology (DH) domain |
| Shipping Condition: | Blue Ice |
| Unit(s): | 250 ug |
| Host name: | Sheep |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | |
| Application: | IP, WB |
Description
Description: The immunogen used for the anti-human Vav-2 DPH region was a his tagged fusion protein Vav-2 corresponding to amino acid sequence 287-578 and having a molecular weight of 100kD. Vav-2 has a molecular weight of 100 kD and shares over a 50% homology at the amino acid and nucleic acid level with Vav. Vav-2 is a ubiquitously expressed structural homolog of the Vav protooncogene that is expressed preferentially in hematopoetiic cells. Both proteins are comprised of a Dbl- homology ((DH) domain with guanosine nucleotide exchange (GEF) activity exclusively directed towards Rho/Rac GTPases, a pleckstrin homology (PH) domain, a calponin-homology (CH) region, an acidic domain (AD) a zinc finger butterfly motif, two SH3 regions and one SH2 domain. GEF activity of RhoA family G proteins is induced by tyrosine phosphorylation in wild type vav-2, and is constitutively activated in N terminus deleted oncogene forms. Constitutive expression of a Vav-2 oncoprotein may result in morphological alterations including highly enlarged cells in which karyokinesis and cytokenesis frequently are uncoupled.
Additional Text
Gene ID
7410
Gene Name
VAV2
Uniprot ID
P52735
Purification
Ammonium Sulfate Precipitated
Antibody Clonality
Polyclonal
Application Notes
Western Blot 1:2500 -1:5000. Immunoprecipitation -Empirical Dilutions should be titrated for each application to obtain the optimal antibody titer.
Storage Note
Product should be stored at -20C. Aliquot to avoid freeze/thaw cycles
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