SR Proteins (Non-snRNP Splicing Factor) (MaxLight 550)
Catalogue Number: S6570-ML550-USB
| Manufacturer: | United States Biological |
| Physical state: | Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™550. |
| Type: | Monoclonal Primary Antibody - Conjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Mouse |
| Clone: | 3G269 |
| Isotype: | IgG1, kappa |
| Immunogen: | Purified, de-phosphorylated bovine Srp55 and Drosophila p55-GST. The epitope has been mapped to a 40 amino acid polypeptide composed almost exclusively of arginine alternating with glutamate and aspartate. |
| Application: | ICC, IF, WB, FLISA |
Description
Description: MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000. SR proteins are a highly conserved family of arginine/serine rich, spliceosome associated phosphoproteins essential for metazoan pre-mRNA splicing. SR proteins appear to act early in splicing by promoting splice site recognition and spliceosome assembly. SR proteins also play a regulatory role, because they can determine alternative splice site usage in vivo and in vitro-a particularly interesting example is the regulation of adenovirus alternative RNA splicing by dephosphorylation of SR proteins. SR proteins appear to be recruited from nuclear "speckles", in which they are concentrated, to sites of transcription in order to spatially coordinate transcription and pre-mRNA splicing within the cell nucleus. The epitope detected by the antibody has been termed the "alternating arginine domain" and is composed almost exclusively of argnine alternating with glutamate and aspartate. Antibody targets are predominantly nuclear, nonnucleolar, and are localized to active sites of polymerase II transcription. Epitope is conserved in metazoans.
Additional Text
Specificity
Recognizes a sub-set of the non-snRNP splicing factors termed SR proteins. Detects SRp75, SRp55, SRp40 and SRp20 but not SRp30a or b (ASF/SF2, SC-35) proteins. Recognizes ~20 distinct nuclear proteins including the U1 70K component of the U1 snRNP and both subunits of U2AF1. Species Crossreactivity (Western Blot): chicken, human, mouse, rabbit, canine, rat, Xenopus. Reactivity has not been detected with yeasts.
Purification
Protein A purified
Antibody Clonality
Monoclonal
S6570-ML550 Datasheet
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