STAT 5, pan (Signal Transducer and Activator of Transcription 5, pan) (MaxLight 550)
Catalogue Number: S7972-07B-ML550-USB
| Manufacturer: | United States Biological |
| Physical state: | Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™550. |
| Type: | Monoclonal Primary Antibody - Conjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Mouse |
| Clone: | 3G277 |
| Isotype: | IgG1, kappa |
| Immunogen: | A peptide corresponding to a conserved amino acid sequence found near the carboxy-terminus of mouse STAT 5. The peptide encompasses the conserved C-terminal tyrosine phosphorylation site (Y-694) of mouse STAT 5. |
| Application: | ICC, IF, WB, FLISA |
Description
Description: MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000. The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells.
Additional Text
Specificity
Reacts specifically with both mouse STAT5a and STAT5b proteins. Can be used to detect both the un-phosphorylated and phosphorylated forms of STAT5. Does not crossreact with other endogenous STAT isoforms. Species Crossreactivity: Human and rat. Lysates Tested: Cell lysates derived from NIH 3T3 cells, A431 cells, MCF-7 cells, HeLa cells, and 293 cells transfected with either a STAT5 expression vector alone or together with a Jak1 expression vector.
Purification
Affinity Purified
Antibody Clonality
Monoclonal
S7972-07B-ML550 Datasheet
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