Catalogue Number: S9687-SEL
| Manufacturer: | Selleck Chemicals |
| Shelf Life: | 36 months |
| Molecular Formula: | C24H16Cl2O7 |
| Type: | Probes |
| Alias: | 2',7'-Dichlorodihydrofluorescein diacetate |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 mg, 25 mg, 10 mM/1 ml, 1 g |
H2DCFDA (DCFH-DA) is a cell-permeable probe used to detect intracellular reactive oxygen species (ROS) production. It is de-esterified intracellularly and turns to highly fluorescent 2',7'-dichlorofluorescein upon oxidation. It is used in sensitive and rapid quantitation of oxygen-reactive species in response to oxidative metabolism; microplate assay for detecting oxidative products in phagocytic cells, and quantitative multiwell myeloid differentiation assay.(Ex/Em=488/525 nm)
CC(=O)OC1=CC2=C(C=C1Cl)C(C3=C(O2)C=C(OC(C)=O)C(=C3)Cl)C4=C(C=CC=C4)C(O)=O
3 years -20°C(in the dark) powder