Sheep anti Mouse IgG2a (1b allotype specific), conjugated with Horseradish peroxidase

Catalogue Number: SHAM/IGG2A(1B)/PO-NMB

Manufacturer:	Nordic MuBio
Type:	Polyclonal Secondary Antibody - Conjugated
Shipping Condition:	RT
Unit(s):	1 ml
Host name:	Sheep
Clone:
Isotype:
Immunogen:

Overview

Description

Description: The reactivity of the antiserum is restricted to the Fc portion of the IgG2a molecule. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against mouse serum of the allelic type Igh-1b, including C57Bl and SJL/J, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgG2a(1b). No precipitation reaction is obtained with purified IgG2a(1a) (Balb/C, CBA, AKR and NZB), other IgG subclasses, IgA, IgG/Fab fragments or other serum proteins. In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG2a(1b) at the cellular an subcellular level. In non-isotopic assay methodology (e.g. ELISA and Western blotting) to identify and measure IgG2a(1b) in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. Its optimum working dilution should be established by titration. Excess labelled antibody may by itself cause high unspecific background staining or interfere with the specific signal.

Additional Text

Antibody Clonality

Polyclonal

Storage Note

The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

Application Notes

ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.

Price

£520.00

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