Sheep anti Rat IgG2b (subclass specific)
Catalogue Number: SHARA/IGG2B-NMB
| Manufacturer: | Nordic MuBio |
| Type: | Polyclonal Secondary Antibody - Unconjugated |
| Shipping Condition: | RT |
| Unit(s): | 1 ml |
| Host name: | Sheep |
| Clone: | |
| Isotype: | |
| Immunogen: |
Description
Description: The reactivity of the antiserum is directed to the Fc portion of the subclass IgG2b. It does not react with other subclasses of IgG, IgG/Fab fragments, IgM and IgA or any non-Ig protein in rat serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgG2b in rat serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of rat IgG2b from serum or plasma.
Additional Text
Antibody Clonality
Polyclonal
Storage Note
The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.
Application Notes
Precipitation assays. In immunoelectrophoresis use 2 µl or equivalent against 120 µl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
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