Swine anti Human Hepatitis B associated antigen

Catalogue Number: SWAHU/HAA-NMB

Manufacturer:Nordic MuBio
Type:Polyclonal Primary Antibody - Unconjugated
Shipping Condition:RT
Unit(s): 1 ml
Host name: Pig
Clone:
Isotype:
Immunogen:

Description

Description: The defined antibody specificity is to the hepatitis associated antigen as tested in counterelectrophoresis and double radial immunodiffusion against HAA positive human serum. The product does not react with normal human serum or plasma. In precipitating techniques as counteroelectrophoresis and double radial immunodiffusion (Ouchterlony) to identify the presence of hepatitis associated antigen.

Additional Text

Antibody Clonality

Polyclonal

Storage Note

The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.

Application Notes

Precipitation assays. In counter electrophoresis use opposite holes at a distance of 4mm with 6 µl antiserum and serum samples. In double radial immunodiffusion use a rosette arrangement with 10 µl antiserum in 3 mm diameter center well and 2 µl plasma samples (neat and serially diluted) in 2 mm diameter peripheral wells. The distance between the wells should be 2-3 mm. In case the concentration of the protein component is significantly increased or reduced, the required equivalence between the reactants can be establish by increasing the amount of antiserum or antigen respectively.