Swine anti Human IgE (Fc specific)
Catalogue Number: SWAHU/IGE(FC)-NMB
| Manufacturer: | Nordic MuBio |
| Type: | Polyclonal Secondary Antibody - Unconjugated |
| Shipping Condition: | RT |
| Unit(s): | 1 ml |
| Host name: | Pig |
| Clone: | |
| Isotype: | |
| Immunogen: |
Description
Description: The reactivity of the antiserum is restricted to the Fc part of the IgE molecule. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against normal human serum containing monoclonal IgE a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgE. No precipitation reaction is obtained with purified IgG, IgM, and IgG/Fab fragments. In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgE in human serum or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of human IgE from serum or plasma. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such use labelled and unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity and performance requirements are available.
Additional Text
Antibody Clonality
Polyclonal
Storage Note
The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.
Application Notes
Precipitation assays. In immunoelectrophoresis use 2 µl serum or equivalent against 120 µl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 µl antiserum in 3 mm diameter center well and 2 µl serum samples (neat and serially diluted in 2 mm diameter peripheral wells.
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations