Swine anti Human IgG (Fc specific)
Catalogue Number: SWAHU/IGG(FC)-NMB
| Manufacturer: | Nordic MuBio |
| Type: | Polyclonal Secondary Antibody - Unconjugated |
| Shipping Condition: | RT |
| Unit(s): | 1 ml |
| Host name: | Pig |
| Clone: | |
| Isotype: | |
| Immunogen: |
Description
Description: The reactivity of the antiserum is directed to the Fc subunit of the IgG molecule. In immunoelectrophoresis and double radial immunodiffusion, using various antiserum concentrations against normal human plasma and serum, a characteristic precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgG. No reaction is obtained with purified IgA, IgM and IgG/Fab fragments or any other serum protein.
Additional Text
Antibody Clonality
Polyclonal
Storage Note
The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.
Application Notes
Precipitation assays. In immunoelectrophoresis use 2 µl or equivalent against 120 µl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 µl in a 3 mm diameter center well and 2 µl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells.
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