Swine anti Rabbit IgG (heavy and light chains)

Catalogue Number: SWAR/IGG(H+L)/7S-NMB

Manufacturer:	Nordic MuBio
Type:	Polyclonal Secondary Antibody - Unconjugated
Shipping Condition:	RT
Unit(s):	10 mg
Host name:	Pig
Clone:
Isotype:
Immunogen:

Overview

Description

Description: The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in rabbit serum, as tested by immunoelectrophoresis and double radial immunodiffusion. As unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user's own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.

Additional Text

Antibody Clonality

Polyclonal

Storage Note

The lyophilized IgG fraction is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Application Notes

Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting.

Price

£209.00

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