Chicken anti Indole-3-butyric acid

Catalogue Number: T0158P-NMB

Manufacturer:Nordic MuBio
Type:Polyclonal Primary Antibody - Unconjugated
Shipping Condition:RT
Unit(s): 1 ml
Host name: Chicken
Clone:
Isotype: IgY
Immunogen: BSA-Indole-3-butyric acid conjugate
Application: ELISA, AC

Description

Description: Indole-3-butyric acid (IBA) is a plant hormone belonging to the group of auxins. It is used to stimulate root formation in plant cuttings.

Additional Text

Antibody Clonality

Polyclonal

Application Notes

The antibody may be used in ELISA and Immuno-affinity-chromatography (IAC). Samples may include cutting medium samples and extraction of these samples may be performed using PBS-10% methanol, followed by neutralisation of the extract. Suggested dilution 1:12,800 in indirect ELISA (this titer is defined as the dilution that gives 50 % of the absorbance from the maximum absorbance when tested with ELISA). Plates are coated with 400 ng/ml ovalbumine-conjugated indole-3-butyric acid. HRP-conjugated anti-chicken IgY should be used as a tracer in a 1:8,000 dilution.

Storage Note

Store at 4 °C or -20 °C. The working antibody solution is stable for at least 7 days at 4 °C. Precautions should be taken for storage for longer periods. Problems of longterm stability may occur with highly diluted solutions. For long storage purposes in solution the addition of sodium azide to 0.02 % is advised with the appropriate precautions of use.