Anti-Mouse TIM-1 (CD365) - Purified in vivo PLATINUM™ Functional Grade

Catalogue Number: T744-LET

Manufacturer:	Leinco Technologies, Inc
Type:	Monoclonal Primary Antibody - Unconjugated
Shipping Condition:	Blue Ice
Unit(s):	1 mg, 100 mg, 25 mg, 5 mg, 50 mg
Host name:
Clone:	RMT1-10
Isotype:
Immunogen:
Application:	ELISA, FA, Agon

Overview

Description

Description: TIM-1 is a member of the T cell immunoglobulin mucin gene family and encodes a cell-surface glycoprotein consisting of an immunoglobulin variable-region-like domain (IgV), mucin-like domain, transmembrane domain, and intracellular tail1,2. TIM-1 promotes activated T cell survival and plays a role in immunopathology2. TIM-1 is also expressed by tubules injured by renal disease and in this context is known by its alternative name, kidney injury molecule-1 (KIM-1). Renal expression of TIM-1 is associated with dedifferentiation of epithelial cells and is involved in the phagocytosis of apoptotic debris. TIM-1 also plays a role in immune responses and is linked to airway hypersensitivity in mice and asthma, eczema, and rheumatoid arthritis in humans1. TIM-1 was first identified as a hepatitis A virus cellular receptor1. TIM-4 is a natural ligand of TIM-1. RMT1-10 was generated by immunizing SD rats with full-length TIM-1-Ig containing both the IgV and mucin domains1. Lymph nodes were fused with P3U1 myeloma cells. Resulting hybridomas were screened for binding to mouse TIM-1-transfected CHO cells. RMT1-10 binds to full-length and mucinless forms of TIM-1, suggesting its epitope resides in the IgV domain1. Blocking TIM-1 in culture via the addition of RMT1-10 results in reduced T cell proliferation, IFN-γ and IL-17 inhibition, and induction of the Th2 cytokines IL-4 and IL-101. Additionally, RMT1-10 significantly improves the survival rate of corneal allografts3,4 and attenuates atherosclerosis development and progression by expanding atheroprotective B1a cells5 in mice.

Additional Text

Concentration

5 mg/ml

Purification

Purified

Antibody Clonality

Monoclonal

Storage Note

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.

Price

£200.00

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