Anti-Mouse TIM-2 - Purified in vivo PLATINUM™ Functional Grade
Catalogue Number: T763-LET
| Manufacturer: | Leinco Technologies, Inc |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg, 100 mg, 25 mg, 5 mg, 50 mg |
| Host name: | |
| Clone: | RMT2-25 |
| Isotype: | |
| Immunogen: | |
| Application: | FC, IP, WB, FA, Agon |
Description
Description: The T cell/transmembrane, immunoglobulin, and mucin (TIM) family plays a critical role in immune response, transplant tolerance, autoimmunity, and allergy and asthma regulation1. The TIM family consists of eight members, including TIM-2 which is only found in rodent genomes. TIM-2 is likely a gene duplication of TIM-1. TIM-2 is a type I cell surface glycoprotein that has an N-terminal immunoglobulin-like (Ig) domain, a mucin domain with O- and N-linked glycosylations, a single transmembrane domain, and a cytoplasmic region with tyrosine phosphorylation motif. Unlike other TIM family proteins, TIM-2 does not bind PtdSer. TIM-2 is a receptor for H-ferritin. TIM-2 functions as a negative regulator of T cell activation and Th2 responses. RMT2-25 was generated by immunizing Sprague Dawley rats with a TIM-2-Ig chimera protein consisting of murine TIM-2-Ig (extracellular domain residues 1-230) and the Fc portion of mouse IgG2a2 . LN cells were fused with P3U1 myeloma cells. The RMT2-25 hybridoma was selected using flow cytometry, based on reactivity to mouse TIM-2 transfected cells, but not parental cells. RMT2-25 inhibits the binding of H-ferritin to TIM-2. RMT2-25 also significantly enhances B cell proliferation and activation in vitro by agonistic stimulation of TIM-2.
Additional Text
Concentration
5 mg/ml
Purification
Purified
Antibody Clonality
Monoclonal
Storage Note
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.
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