Tubulin (MaxLight 650)
Catalogue Number: T9154-04-ML650-USB
| Manufacturer: | United States Biological |
| Physical state: | Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650. |
| Type: | Monoclonal Primary Antibody - Conjugated |
| Alias: | B-ALPHA-1|hum-a-tub1|hum-a-tub2|LIS3|TUBA3|tubulin alpha-1A chain|tubulin alpha-3 chain|tubulin B-alpha-1|tubulin, alpha, brain-specific |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rat |
| Clone: | 2Q1105 (YL1/2) |
| Isotype: | IgG2a |
| Immunogen: | Yeast tubulin |
| Application: | IP, WB, RIA, IHC, FLISA |
Description
Description: MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. Tubulins, major components of the cellular cytoskeleton, form microtubules that are critical for cellular structure, migration, cell division and intracellular trafficking. There are at least three types of tubulins, alpha, beta and gamma. Each has a molecular weight of approximately 55kD. Microtubules are large structures composed of alternating alpha and beta subunits. Compounds that block microtubule formation, such as Taxol and the vinca alkaloids, inhibit cell division and are effective anti-cancer agents. The epitope recognized by this antibody has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C-terminus, with the two adjacent amino acids being negatively charged (represented by Gly-Gly-Tyr in Tyr-Tubulin). The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized actin.
Additional Text
Gene Name
TUBA1A
Uniprot ID
Q71U36
Gene ID
7846
Specificity
Recognizes the alpha subunit of human tubulin, specificity binding to so called Tyr-Tubulin at ~55kD, which is produced by post translational modification of tubulin. Species crossreactivity: mouse and rat.
Purification
Protein A purified
Antibody Clonality
Monoclonal
T9154-04-ML650 Datasheet
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