Catalogue Number: TMI-CHI-1-CENTRI-IDY
Manufacturer: | Idylle Labs |
Type: | Coverslips |
Shipping Condition: | RT |
Unit(s): | 1 slide + 1 kit |
Application: | Mcrspy |
Description: Chitozen is a chitosan-coated coverslip immobilizing bacteria for microscopy without inducing bacteriostatic effects. It is compatible with 6-channel sticky slides for flow experiments. It is very helpful if you want to: -Image bacteria both still and alive under the microscope-Maintain bacteria in a same focal plane for imaging while preserving their physiology -Renew culture medium, change growth condition (e.g. antibiotics, chemicals, inhibitors) during the experiment and directly observe, in real-time, the bacteria new comportment under the microscopeKit contentsChitozen coverslips are compatible with 6-channel sticky slides to work in a closed system in static or dynamic flow conditions. Order you coverslips alone if you need to work in an open system (i.e. for AFM imaging) or to place them on depression slides.1-coverslips with microfluidic channelCentrifugation pack: the Chitozen coverslips can be centrifuged using a rack compatible with standard microscope slide (25mmx75mm) dimensions. It includes: > µ-Slide Microscopy Rack (ibidi > Magnetic Lid for Microscopy Rack > Clamp & adapter for sticky slidesApplicationsAssay compatibility: > Fluorescence> Co-cultures (bacterial predators, immune cells)> Addition of external factors (e.g. antibiotics, chemicals, inhibitors)> Static or dynamic conditionsImaging modes: > phase-contrast, > epifluorescence, > confocal, > super-resolution microscopy, > atomic force microscopy (AFM) - documentation & example pictures available on requestExperimental outputs:> Behavioural changes: growth, elongation, cell division, fitness, colony/biofilm formation, etc> Single molecule imaging Chitozen is efficient with the following bacteria: > E. coli> Bacillus subtilis> Caulobacter crescentus> Corynebacterium glutamicum> Helicobacter pylori > Mycobacterium smegmatis> Myxococcus xanthus> Pseudomonas aeruginosa> Pseudomonas fluorescens> Salmonella> Staphylococcus aureus> Vibrio choleraeTechnical specificationsMaterial: glassFormat: 75.0 x 25.0 mmThickness: 0.170 mm / 1.5HLifetime: up to 12 months at room temperature, shielded from direct sunlight
Functionalized microscope coverslips for bacteria live imaging