Mouse anti Human Calpain I, II (small subunit), conjugated with agarose
Catalogue Number: X1011-NMB
| Manufacturer: | Nordic MuBio |
| Type: | Monoclonal Primary Antibody - Conjugated |
| Alias: | Mouse anti calpain I, II small subunit, p30, agarose conjugate |
| Shipping Condition: | RT |
| Unit(s): | 1 ml |
| Host name: | Mouse |
| Clone: | 28F3 |
| Isotype: | IgG1 |
| Immunogen: | Hybridoma produced by the fusion of splenocytes from mice immunized with human calpain with mouse myeloma. |
| Application: | IP, WB |
Description
Description: The calpains are calcium-dependent cysteine proteases that are widely expressed in mammalian systems. Both m-calpain (calpain II) and µ-calpain (calpain I) are composed of an 80 kD subunit and a 30 kD subunit. Whereas the 30 kDa subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca ++ requirements that are suggested by their names. Whereas m-calpain requires millimolar (mM) levels of calcium, µ-calpain is active at micromolar (µM) concentrations of Ca ++. In addition to the ubiquitously expressed m- and µcalpains, some tissue-specific calpains have been identified. The calpains appear to serve multiple physiological roles, and ideas concerning the functions of these enzymes are in a state of rapid flux.
Additional Text
Gene Name
CAPN2
Gene ID
824
Uniprot ID
P17655
Purification
Protein A/G Purified
Antibody Clonality
Monoclonal
Application Notes
Application: Use at 0.5-1 ug/ml for ELISA and western blot, 1-2ug/ml for immunoprecipitation.
Storage Note
Product should be stored at -20C. Aliquot to avoid freeze/thaw cycles
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