Mouse anti Phosphotyrosine, conjugated with biotin

Catalogue Number: X1016-NMB

Manufacturer:	Nordic MuBio
Type:	Monoclonal Primary Antibody - Conjugated
Alias:	Mouse anti phosphotyrosine, PY20, Biotin Conjugate
Shipping Condition:	RT
Unit(s):	100 ug
Host name:	Mouse
Clone:	PY20
Isotype:	IgG2b
Immunogen:
Application:	IP, WB, IHC, EIA

Overview

Description

Description: The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine-phosphorylated proteins biochemical pathways in which they function.

Additional Text

Purification

Protein A/G Purified

Antibody Clonality

Monoclonal

Application Notes

The biotin conjugate of clone PY20 anti-phosphotyrosine is especially useful for the detection of phosphotyrosyl proteins in western blotting, immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would produce unacceptable background signals. In addition, the strength of the biotin-streptavidin interaction and the immense variety of streptavidin-coupled probes available make PY20-biotin a very versatile reagent. A dilution of 1:5,000 is suggested for western blot or ELISA, 1:500 for immunohistochemistry or immunocytochemistry.

Storage Note

Product should be stored at -20C. Aliquot to avoid freeze/thaw cycles

Price

£278.00

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