Mouse anti Phosphotyrosine, conjugated with peroxidase

Catalogue Number: X1018S-NMB

Manufacturer:	Nordic MuBio
Type:	Monoclonal Primary Antibody - Conjugated
Alias:	MoMouse anti phosphotyrosine, PY20, HRP conjugate, Horseradish peroxidase conjugate
Shipping Condition:	RT
Unit(s):	100 ug
Host name:	Mouse
Clone:	PY20
Isotype:	IgG2b
Immunogen:	Hybridoma produced from Balb/C mice immunized with phosphotyrosine coupled to carrier protein.
Application:	IP, WB, IHC, EIA

Overview

Description

Description: The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine-phosphorylated protein biochemical pathways in which they function. The peroxidase conjugate of clone PY20 anti-phosphotyrosine is especially useful for the detection of phosphotyrosyl proteins in western blotting, immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would produce undesirable background signals.

Additional Text

Purification

Protein A/G Purified

Antibody Clonality

Monoclonal

Application Notes

A dilution of 1:2,000 is suggested for western blot or ELISA, 1:500 for immunoprecipitation, immunohistochemistry, or immunocytochemistry.

Storage Note

Product should be stored at -20C. Aliquot to avoid freeze/thaw cycles

Price

£278.00

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