Mouse anti Phosphotyrosine, conjugated with peroxidase
Catalogue Number: X1018XS-NMB
| Manufacturer: | Nordic MuBio |
| Type: | Monoclonal Primary Antibody - Conjugated |
| Alias: | Mouse anti phosphotyrosine, PY20, HRP conjugate, Horseradish peroxidase conjugate |
| Shipping Condition: | RT |
| Unit(s): | 50 ug |
| Host name: | Mouse |
| Clone: | PY20 |
| Isotype: | IgG2b |
| Immunogen: | |
| Application: | IP, WB, IHC, EIA |
Description
Description: The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years, While the phosphorylation of specific tyrosine residues has be shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved invaluable to the study of tyrosine-phosphorylated proteins and the biochemical pathways in which they fuction. The HRP conjugate of PY20 anti-phosphotyrosine is especailly useful for the detection of phosphotyrosyl proteins in western blotting, immunohistochemical and immuncytochemical protcols in situations wherin the use of a secondary antibody would produce undesirable background signals.
Additional Text
Purification
Protein A/G Purified
Antibody Clonality
Monoclonal
Application Notes
Working dilution of 1:2,000 is suggested for western blot or ELISA, 1:500 for immunohistochemistry or immunocytochemistry.
Storage Note
Product should be stored at -20C. Aliquot to avoid freeze/thaw cycles
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations