Mouse anti Human alpha SNAP

Catalogue Number: X1026-EXA

Manufacturer:	ExAlpha
Type:	Monoclonal Primary Antibody - Unconjugated
Alias:	alpha/beta SNAP, mouse anti alpha soluble NSF attachment protein,SNAPA
Shipping Condition:	Blue Ice
Unit(s):	100 ug
Host name:	Mouse
Clone:	15D4
Isotype:	IgG1
Immunogen:	Balb/C mice were immunized with recombinant human alpha -SNAP. The resultant hybridomas were selected for reactivity with the immunogen by ELISA and with the antigen by Western blot.
Application:	IP, WB, EIA

Overview

Description

Description: SNAPs (soluble NSF attachment proteins), acting in concert with SNAREs (SNAP receptors) and the N-ethylmaleimide-sensitive fusion protein (NSF), are required for the fusion of transport vesicles to their target membranes in synaptic transmission, intra-Golgi transport, endosome-to-endosome fusion and transcytotic vesicles-to-plasma membrane transport. Vesicle-to-target membrane docking (initial contact) occurs when the vesicle SNARE binds to its cognate target membrane SNARE. Beta-SNAP (or SNAP in brain) then binds to this docking complex and mediates the binding of NSF and thus the formation of a 20 S fusion particle. It is thought that, once NSF is bound, ATP hydrolysis by NSF initiates the fusion process. Whereas alpha-SNAP is expressed in all mammalian tissues, beta-SNAP is expressed only in brain.

Additional Text

Gene Name

NAPA

Gene ID

8775

Uniprot ID

P54920

Purification

Purified

Antibody Clonality

Monoclonal

Application Notes

Optimal concentration should be evaluated by serial dilutions. Clone 4E4 anti-?SNAP specifically recognizes ?-SNAP as a single band of ~36 kDa on western blot of rat kidney, rat brain and MDBK cells and does not recognize ?-SNAP Application: A dilution of 0.5-5 ug/ml is suggested for western blot and 2-10 ug/ml for immunoprecipitation.

Storage Note

Product should be stored at -20C. Aliquot to avoid freeze/thaw cycles

Price

£322.00

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