Collagen Substrate, FITC Conjugate

Catalogue Number: X1098-EXA

Manufacturer:ExAlpha
Type:Protein - Conjugated
Alias:Extracted from bovine skin
Shipping Condition:Blue Ice
Unit(s): 10 mg
Host name: Bovine
Clone:
Isotype:
Immunogen: Bovine skin
Application: FA

Description

Description: FITC-labeled bovine type I Collagen Substrate is an excellent substrate for examining collagenase activity. This labelled substrate is highly purified and telo-peptide free and is supplied lyophilized. To minimize background levels in collagenase assays, FITC-labeled collagen has been enzymatically pre-treated and further purified by ion-exchange chromatography. FITC-labeled collagen can also be used as a substrate for cell culture. Collagen degradation products in the culture supernatants can be directly determined by measured the flourescence at 520 nm (Emission)/490 nm (Excitation).

Additional Text

Application Notes

Used for the assay of mammalian collagenase activity. To heat denature collagen for preparing a substrate for gelatinase, transfer collagen solution to a brown glass bottle and heat to 80C.

Storage Note

Product should be stored at -20C. Aliquot to avoid freeze/thaw cycles