Mouse anti Human p21 WAF-1
Catalogue Number: X1158M-NMB
| Manufacturer: | Nordic MuBio |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Shipping Condition: | RT |
| Unit(s): | 100 ug |
| Host name: | Mouse |
| Clone: | WA-1 |
| Isotype: | IgG1 |
| Immunogen: | Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with human p21 protein and mouse myeloma SP2 cells. |
| Application: | ELISA, WB |
Description
Description: p21, also known as wild-type p53-activated factor (WAF-1) or Cdk-interacting protein (CIP1), expression is predominantly induced by p53. This expression is usually in response to DNA damage caused by cytotoxic agents. The expression of p21 interrupts the cell cycle and prevents the replication of the cell containing genomic errors. p21 inhibits Cdk2, Cdk3, Cdk4 and Cdk6 to stop the cell cycle. Contained in the human p21 is a highly conserved cystine-rich region (amino acids 21-60) with a potential zinc finger domain as well as a second conserved region (amino acids 130-164) which contains several putative nuclear localization signals. p21 can also interact with proliferating cell nuclear antigen (PCNA), Gadd45, Cdk2 and cyclins which suggests it may coordinate DNA repair and replication in damaged cells.
Additional Text
Gene ID
1026
Gene Name
CDKN1A
Uniprot ID
P38936
Purification
Protein A/G Purified
Antibody Clonality
Monoclonal
Application Notes
Detects p21 protein by Western blot at 1 to 10 µg/ml. Detects a 21 kDa protein in MCF7 cells. Can be used with either frozen or paraffin embedded tissue sections. Optimal concentration should be evaluated by serial dilutions. Other Applications: ELISA
Storage Note
Product should be stored at -20C. Aliquot to avoid freeze/thaw cycles
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